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平臺編號：bio-68559
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國際編號：CRL-2914?
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細(xì)胞信息： M4A4
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
M4A4
數(shù)量：大量
ATCC Number：CRL-2914?
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
年限：31
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
器官來源：其他
規(guī)格：0.1ml Designations: M4A4
Depositors: ?D Tarin
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: See note in the comments below
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Receptors:epidermal growth factor (EGF), expressed
Oncogene:c-myc; Ras; p53
Antigen Expression:CD44; Homo sapiens, expressed
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 11,12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18
Age: 31
Gender: female
Ethnicity: Caucasian
Comments:The parental cell lines CRL-2914 (M4A4) and CRL-2918 (NM2C5) were derived from the human breast cancer cell line, MDA-MB-435. [PubMed: 7683291]. M4A4 is highly metastatic in immuno-deprived mice, while NM2C5 is weakly or virtually non-metastatic. These well characterized, tumorigenic human isogenic cell lines have dramatically opposite metastatic phenotypes and are ideal for metastatic studies.M4A4 LM3-4 CL 16 GFP (CRL-2917) cell line was derived from a third generation lung metastasis after inoculation of M4A4 GFP cells in a nude mouse mammary gland. The M4A4 GFP (CRL-2915) was developed by the transduction of the GFP gene into M4A4 (CRL-2914) cell line. One of the isolated cell lines, M4A4 LM3-2 GFP (CRL-2916) was derived from a second lung metastasis.
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line. [16173089 ] [16173090 ] [16173091 ] [16173092 ] [16173093 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Protocol: To avoid phenotypic drift it is recommended to make frozen aliquots of the cells and use each aliquot for only 10 passages.
Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 30 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
phosphate-buffered saline:ATCC 30-2200
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
derived from same cell line:ATCC CRL-2915
derived from same cell line:ATCC CRL-2918
derived from same cell line:ATCC CRL-2919
derived from same cell line:ATCC CRL-2916
derived from same cell line:ATCC CRL-2917
References: 90473: Bao L, et al. Correlation of VLA-4 integrin expression with metastatic potential in various human tumour cell lines. Differentiation 52: 239-246, 1993. PubMed: 7683291
90474: Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541
92860: Goodison S, et al. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9: 3808-3814, 2003. PubMed: 14506175
92861: Montel V, et al. Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model. Am. J. Pathol. 166: 1565-1579, 2005. PubMed: 15855655
92862: Suzuki M, et al. Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency. Am. J. Pathol. 169: 673-681, 2006. PubMed: 16877365
92863: Montel V, et al. Tumor-stromal interactions reciprocally modulate gene expression patterns during carcinogenesis and metastasis. Int. J. Cancer 119: 251-263, 2006. PubMed: 16482564
92864: Goodison S, et al. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet. Cytogenet. 156: 37-48, 2005. PubMed: 15588854
92865: Hayashi K, et al. Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int. J. Oncol. 22: 623-629, 2003. PubMed: 12579317
92888: Tarin DTumor metastasisIn: Tarin DOxford Textbook of PathologyOxford, United KingdomOxford University Press607-663, 1992
16173089: Ross DT, et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174.
16173090: Sellappan S, et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res. 64: 3479-3485, 2004. PubMed: 15150101.
16173091: Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052.
16173092: Ellison G, et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931.
16173093: Rae JM, et al., MDA-MB-435 cells are derived from M14 Melanoma cells - a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res. Treat. 104:13-19, 2007. PubMed: 17004106.
ATCC Number：CRL-2914?
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
年限：31
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
器官來源：其他
規(guī)格：0.1ml Designations: M4A4
Depositors: ?D Tarin
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: See note in the comments below
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Receptors:epidermal growth factor (EGF), expressed
Oncogene:c-myc; Ras; p53
Antigen Expression:CD44; Homo sapiens, expressed
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 13
D5S818: 11,12
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 16,18
Age: 31
Gender: female
Ethnicity: Caucasian
Comments:The parental cell lines CRL-2914 (M4A4) and CRL-2918 (NM2C5) were derived from the human breast cancer cell line, MDA-MB-435. [PubMed: 7683291]. M4A4 is highly metastatic in immuno-deprived mice, while NM2C5 is weakly or virtually non-metastatic. These well characterized, tumorigenic human isogenic cell lines have dramatically opposite metastatic phenotypes and are ideal for metastatic studies.M4A4 LM3-4 CL 16 GFP (CRL-2917) cell line was derived from a third generation lung metastasis after inoculation of M4A4 GFP cells in a nude mouse mammary gland. The M4A4 GFP (CRL-2915) was developed by the transduction of the GFP gene into M4A4 (CRL-2914) cell line. One of the isolated cell lines, M4A4 LM3-2 GFP (CRL-2916) was derived from a second lung metastasis.
Note: Recent studies have generated questions about the origin of the parent cell line, MDA-MB-435. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line. [16173089 ] [16173090 ] [16173091 ] [16173092 ] [16173093 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Protocol: To avoid phenotypic drift it is recommended to make frozen aliquots of the cells and use each aliquot for only 10 passages.
Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.
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Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentration between 8 X 10(4) and 1 X 10(5) cells/sq. cm.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 2 to 3 times a week
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 30 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
phosphate-buffered saline:ATCC 30-2200
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402
derived from same cell line:ATCC CRL-2915
derived from same cell line:ATCC CRL-2918
derived from same cell line:ATCC CRL-2919
derived from same cell line:ATCC CRL-2916
derived from same cell line:ATCC CRL-2917
References: 90473: Bao L, et al. Correlation of VLA-4 integrin expression with metastatic potential in various human tumour cell lines. Differentiation 52: 239-246, 1993. PubMed: 7683291
90474: Urquidi V, et al. Contrasting expression of thrombospondin-1 and osteopontin correlates with absence or presence of metastatic phenotype in an isogenic model of spontaneous human breast cancer metastasis. Clin. Cancer Res. 8: 61-74, 2003. PubMed: 11801541
92860: Goodison S, et al. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9: 3808-3814, 2003. PubMed: 14506175
92861: Montel V, et al. Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model. Am. J. Pathol. 166: 1565-1579, 2005. PubMed: 15855655
92862: Suzuki M, et al. Dormant cancer cells retrieved from metastasis-free organs regain tumorigenic and metastatic potency. Am. J. Pathol. 169: 673-681, 2006. PubMed: 16877365
92863: Montel V, et al. Tumor-stromal interactions reciprocally modulate gene expression patterns during carcinogenesis and metastasis. Int. J. Cancer 119: 251-263, 2006. PubMed: 16482564
92864: Goodison S, et al. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotype-specific chromosomal rearrangements. Cancer Genet. Cytogenet. 156: 37-48, 2005. PubMed: 15588854
92865: Hayashi K, et al. Differential effects of retinoic acid on the growth of isogenic metastatic and non-metastatic breast cancer cell lines and their association with distinct expression of retinoic acid receptor beta isoforms 2 and 4. Int. J. Oncol. 22: 623-629, 2003. PubMed: 12579317
92888: Tarin DTumor metastasisIn: Tarin DOxford Textbook of PathologyOxford, United KingdomOxford University Press607-663, 1992
16173089: Ross DT, et al. Systematic variation in gene expression patterns in human cancer cell lines. Nature Genetics 24: 227-235, 2000. PubMed: 10700174.
16173090: Sellappan S, et al. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line. Cancer Res. 64: 3479-3485, 2004. PubMed: 15150101.
16173091: Rae JM, et al. Common origins of MDA-MB-435 cells from various sources with those shown to have melanoma properties. Clin. Exp. Metastasis 21: 543-552, 2004. PubMed: 15679052.
16173092: Ellison G, et al. Further evidence to support the melanocytic origin of MDA-MB-435. Mol. Pathol. 55: 294-299, 2002. PubMed: 12354931.
16173093: Rae JM, et al., MDA-MB-435 cells are derived from M14 Melanoma cells - a loss for breast cancer, but a boon for melanoma research. Breast Cancer Res. Treat. 104:13-19, 2007. PubMed: 17004106.
