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平臺(tái)編號(hào)：bio-68560
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國(guó)際編號(hào)：CRL-2048?
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細(xì)胞信息： BCE C/D-1b
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
BCE C/D-1b
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
組織來(lái)源：cornea
ATCC Number：CRL-2048?
器官來(lái)源：眼
細(xì)胞類型：內(nèi)皮細(xì)胞
年限：adult
細(xì)胞形態(tài)：內(nèi)皮樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：牛
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：0.1ml Designations: BCE C/D-1b
Depositors: ?AH Nicholas, C Bethea
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Bos taurus
Morphology:endothelial
Source: Organ: eye
Tissue: cornea
Cell Type: endothelial
Cellular Products:extracellular matrix (ECM) proteins
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Gender: male
Comments:This line was established from explants of normal adult bovine corneas.
The cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes.
The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.
The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin, add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22458: Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215
22690: Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171
數(shù)量：大量
組織來(lái)源：cornea
ATCC Number：CRL-2048?
器官來(lái)源：眼
細(xì)胞類型：內(nèi)皮細(xì)胞
年限：adult
細(xì)胞形態(tài)：內(nèi)皮樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：牛
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
規(guī)格：0.1ml Designations: BCE C/D-1b
Depositors: ?AH Nicholas, C Bethea
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Bos taurus
Morphology:endothelial
Source: Organ: eye
Tissue: cornea
Cell Type: endothelial
Cellular Products:extracellular matrix (ECM) proteins
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Gender: male
Comments:This line was established from explants of normal adult bovine corneas.
The cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes.
The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.
The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin, add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22458: Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215
22690: Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171
