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平臺編號：bio-68561
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國際編號：CRL-1606?
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細胞信息： HFN 7.1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
HFN 7.1
器官來源：脾
細胞形態(tài)：淋巴樣
免疫類型：IgG1
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-1606?
數(shù)量：大量
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：48T Designations: HFN 7.1
Depositors: ?RC Schoen, KL Bentley, RJ Klebe
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human fibronectin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Comments:The antibody does not cross-react with other fibronectins tested (26 species including 15 primates).
Mice were immunized with purified human fibronectin.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC HB-39.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
References: 57999: Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125
細胞形態(tài)：淋巴樣
免疫類型：IgG1
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-1606?
數(shù)量：大量
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：48T Designations: HFN 7.1
Depositors: ?RC Schoen, KL Bentley, RJ Klebe
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human fibronectin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Comments:The antibody does not cross-react with other fibronectins tested (26 species including 15 primates).
Mice were immunized with purified human fibronectin.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC HB-39.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
References: 57999: Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125
