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平臺編號：bio-68566
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國際編號：CRL-1535?
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細胞信息： SCC-PSA1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
SCC-PSA1
ATCC Number：CRL-1535?
相關疾?。浩渌膊?br /> 是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：其他
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
品系：129/Sv
規(guī)格：0.5ml Designations: SCC-PSA1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: testes
Strain: 129/Sv
Disease: pluripotent teratocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments:NOTE - NO LIVE CULTURES CAN BE SENT.
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503 ).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.
A somewhat variable percentage will attach.
Change the medium every 2 to 3 days.
Migration of the endodermal layer should begin within 24 to 36 hours after plating.
Do not dislodge cells when changing the medium.
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution to disperse the cells.
Dilute with fresh medium, centrifuge, and resuspend the cells in fresh medium.
Seed cultures with 8 X 10 exp4 cells/sq cm in flasks with irradiated fibroblast feeder layers (e.g., X-irradiated STO cells, ATCC 56-X).
Subculture every 3 days to maintain in an undifferentiated proliferative state.
Related Products:feeder layer cells:ATCC 56-X
References: 1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416
22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624
26077: Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899
相關疾?。浩渌膊?br /> 是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：其他
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
品系：129/Sv
規(guī)格：0.5ml Designations: SCC-PSA1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Organ: testes
Strain: 129/Sv
Disease: pluripotent teratocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments:NOTE - NO LIVE CULTURES CAN BE SENT.
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503 ).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates (embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.
A somewhat variable percentage will attach.
Change the medium every 2 to 3 days.
Migration of the endodermal layer should begin within 24 to 36 hours after plating.
Do not dislodge cells when changing the medium.
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution to disperse the cells.
Dilute with fresh medium, centrifuge, and resuspend the cells in fresh medium.
Seed cultures with 8 X 10 exp4 cells/sq cm in flasks with irradiated fibroblast feeder layers (e.g., X-irradiated STO cells, ATCC 56-X).
Subculture every 3 days to maintain in an undifferentiated proliferative state.
Related Products:feeder layer cells:ATCC 56-X
References: 1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416
22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624
26077: Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899
