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ATCC Number：CRL-1722?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。毫馨土?br /> 數(shù)量：大量
品系：DBA/2
器官來(lái)源：胸腺
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: L5178-R (LY-R)
Depositors: ?JZ Beer
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Organ: thymus
Strain: DBA/2
Disease: lymphoma
Cell Type: chemically induced
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.
The X-ray sensitive L5178-S line (ATCC CRL-1723 ) was derived from this line.
Tested and found negative for ectromelia virus.
Tumorigenic:Yes
Comments:This cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
The cells are resistant to X-rays, but sensitive to ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
After 6 months to 3 years in culture, the line converts to an X-ray sensitive state.
The X-ray sensitive L5178-S line (ATCC CRL-1723 ) was derived from this line.
Tested and found negative for ectromelia virus.
Propagation: ATCC complete growth medium: Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 g/L sodium bicarbonate, 90%; horse serum, 10%

Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 cells/ml and maintain between 3 X 10 exp4 and 7 X 10 exp5 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 1164: Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899
1166: Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332
58059: Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312