品系:B10.BR
ATCC Number:CRL-8079?
數(shù)量:大量
相關(guān)疾?。毫馨土?br />
是否是腫瘤細胞:0
物種來源:小鼠
運輸方式:凍存運輸
生長狀態(tài):懸浮生長
器官來源:脾
細胞形態(tài):淋巴樣
規(guī)格:0.5mg Designations: LBRM-33-1A5
Depositors: ?S Gillis
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Strain: B10.BR
Organ: spleen
Disease: lymphoma
Cellular Products:interleukin-2 (interleukin 2, IL-2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 1 (IL-1)
Comments:The cells produce IL-2 in response to IL-1, and can be used to assay IL-1 activity.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.0025 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Maintain at approximately 8 X 10 exp5 viable cells/ml.
References: 3528: Gillis S. Process for preparing murine interleukin-2 in the presence of interleukin-1 from an interleukin-2 nonproducer malignant cell line and cell line therefor. US Patent 4,404,280 dated Sep 13 1983