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平臺(tái)編號(hào)：bio-68591
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國(guó)際編號(hào)：CRL-1439?
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細(xì)胞信息： clone 9
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
clone 9
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Sprague-Dawley
年限：4 weeks
數(shù)量：大量
器官來(lái)源：肝
ATCC Number：CRL-1439?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。赫?br /> 規(guī)格：0.1ml Designations: clone 9
Depositors: ?ME Kaighn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: liver
Strain: Sprague-Dawley
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1968
Age: 4 weeks
Gender: male
Comments:Clone 9 (K-9) is an epithelial cell line isolated in 1968 from normal liver taken from a young male rat.
The line has been used for studies of in vitro carcinogenesis and is useful clonal assays for screening sera and other nutritional supplements.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 21872: . Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975.
22425: Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：Sprague-Dawley
年限：4 weeks
數(shù)量：大量
器官來(lái)源：肝
ATCC Number：CRL-1439?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。赫?br /> 規(guī)格：0.1ml Designations: clone 9
Depositors: ?ME Kaighn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: liver
Strain: Sprague-Dawley
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1968
Age: 4 weeks
Gender: male
Comments:Clone 9 (K-9) is an epithelial cell line isolated in 1968 from normal liver taken from a young male rat.
The line has been used for studies of in vitro carcinogenesis and is useful clonal assays for screening sera and other nutritional supplements.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 21872: . Gene expression and carcinogenesis in cultured liver. New York: Academic Press; 1975.
22425: Weinstein IB, et al. Growth and structural properties of epithelial cell cultures established from normal rat liver and chemically induced hepatomas. Cancer Res. 35: 253-263, 1975. PubMed: 162864
