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數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-2693?
細(xì)胞形態(tài)：淋巴樣
免疫類型：mouse IgG1 kappa
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
規(guī)格：48T Designations: IgG-3B2
Depositors: ?YK Ho, JL Goldstein
Isotype: mouse IgG1 kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Drosophila sterol regulatory element binding protein (SREBP)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a bacterially produced fusion protein expressing pGST-dSREBP (amino acids 1 to 451). [56310 ]
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of the Drosophila SREBP transcription factor in cultured S2 (Schneider) Drosophila cells.
It can be used in immunoblotting assays to detect Drosophila SREBP and to study SREBP in S2 cells grown in various conditions.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 56310: Seegmiller AC, et al. The SREBP pathway in Drosophila: regulation by palmitate, not sterols. Dev. Cell 2: 229-238, 2002. PubMed: 11832248
57714: Dobrosotskaya IY, et al. Regulation of SREBP processing and membrane lipid production by phospholipids in Drosophila.. Science 296: 879-883, 2002. PubMed: 11988566