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平臺(tái)編號(hào)：bio-68596
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國(guó)際編號(hào)：CRL-2457?
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細(xì)胞信息： PMJ2-PC
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
PMJ2-PC
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
品系：C57BL/6J
ATCC Number：CRL-2457?
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮，少量貼壁
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
規(guī)格：0.2ml Designations: PMJ2-PC
Depositors: ?AV Palleroni
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension; some adherent cells
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Cell Type: peritoneal macrophage; infected with J2 virus
Strain: C57BL/6J
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg -
Gender: female
Comments:PMJ2-PC (ATCC CRL-2457 ) and AMJ2-R (ATCC CRL-2458 ) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48923 ]
Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.
The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages.
Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.
The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.
One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma.
The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. [48934 ]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Twice per week
Scrape off the attached cells and transfer along with the floating cells into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48923: Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328
48934: Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450
品系：C57BL/6J
ATCC Number：CRL-2457?
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮，少量貼壁
細(xì)胞形態(tài)：?jiǎn)魏思?xì)胞/巨噬細(xì)胞
規(guī)格：0.2ml Designations: PMJ2-PC
Depositors: ?AV Palleroni
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension; some adherent cells
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Cell Type: peritoneal macrophage; infected with J2 virus
Strain: C57BL/6J
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg -
Gender: female
Comments:PMJ2-PC (ATCC CRL-2457 ) and AMJ2-R (ATCC CRL-2458 ) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48923 ]
Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines.
The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages.
Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells.
The cells are phagocytic, non-specific esterase positive and are Fc receptor positive.
One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma.
The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. [48934 ]
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Medium Renewal: Twice per week
Scrape off the attached cells and transfer along with the floating cells into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48923: Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328
48934: Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450
