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細胞形態(tài)：成纖維樣
數(shù)量：大量
細胞類型：成纖維細胞
是否是腫瘤細胞：0
物種來源：倉鼠
器官來源：肺
運輸方式：凍存運輸
ATCC Number：CRL-2349?
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Designations: XR-V15B
Depositors: ?G Chu
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cricetulus griseus
Morphology:fibroblast


Source: Organ: lung
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:XR-V15B is a X-ray sensitive Chinese Hamster Lung fibroblast cell line isolated from an ethylnitrosourea (ENU)-mutagenized cell population of V79 cells on the basis of its hypersensitivity to X-rays.
These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen.
Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. Cells will revert to wild type on treatment with azacytidine.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse quickly with 0.25% trypsin, 0.03% EDTA solution, add 1-2 ml additional trypsin solution and allow flasks to set at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
References: 37304: Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631
37306: Errami A, et al. Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants. Mol. Cell. Biol. 16: 1519-1526, 1996. PubMed: 8657125
37309: Smider V, et al. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science 266: 288-291, 1994. PubMed: 7939667
37320: Zdzienicka MZ, et al. Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat. Res. 194: 239-249, 1988. PubMed: 2460764