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生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：HB-8177?
數(shù)量：大量
規(guī)格：48T Designations: 9F12
Depositors: ?University of Rochester Medical Center
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism:human (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against tetanus toxin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Human peripheral blood mononuclear cells (enriched for B cells) were fused with P3X63Ag8.653 myeloma cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate, 5 mcg/ml insulin and 5 mcg/ml transferrin, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1327: Gigliotti F, Insel RA. Protective human hybridoma antibody to tetanus toxin. J. Clin. Invest. 70: 1306-1309, 1982. PubMed: 7174795
3595: Insel RA. Human monoclonal antibodies against bacterial toxins. US Patent 4,689,299 dated Aug 25 1987
58147: Gigliotti F, et al. Reproducible production of protective human monoclonal antibodies by fusion of peripheral blood lymphocytes with a mouse myeloma cell line. J. Infect. Dis. 149: 43-47, 1984. PubMed: 6607295