運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG1
ATCC Number:HB-9509?
是否是腫瘤細(xì)胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
數(shù)量:大量
規(guī)格:1mg Designations: QPN1 22F5 (SCRF 43.2)
Depositors: ?Scripps Clinic and Research Foundation
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with and catalyzes the hydrolysis of certain ester bonds.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 1 mM sodium pyruvate, 1 mM sodium oxaloacetate, 0.1 mM hypoxanthine, 0.032 mM thymidine; penicillin, streptomycin and gentamicin, 97%; bovine serum albumin,
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21999: Lerner RA, et al. Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992