-
平臺(tái)編號：bio-68611
-
國際編號：CRL-11102?
-
細(xì)胞信息： DS-1
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
DS-1
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-11102?
相關(guān)疾?。浩渌膊?br /> 細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：懸浮生長
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG; kappa light chain
規(guī)格：0.3ml Designations: DS-1
Depositors: ?The United States of America
Isotype: IgG; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: lymphangiectasia
Derived from metastatic site: pleural effusion
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 6 (IL-6)
Antigen Expression:HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 -
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9,10
D5S818: 11,14
D7S820: 8,14
THO1: 6,9.3
TPOX: 9,11
vWA: 18,19
Comments:The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.
DS-1 is IL-6 dependent.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: 55-X ).
Medium Renewal: Every 2 to 3 daysATCC Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) cells/ml. Maintain cultures between 1 X 10 (5) and 2 X 10 (6) cells/ml.Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Bioreactive Factors: Growth Factors: interleukin 6 (IL-6)
References: 22116: Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996
ATCC Number：CRL-11102?
相關(guān)疾?。浩渌膊?br /> 細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：懸浮生長
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG; kappa light chain
規(guī)格：0.3ml Designations: DS-1
Depositors: ?The United States of America
Isotype: IgG; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: lymphangiectasia
Derived from metastatic site: pleural effusion
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 6 (IL-6)
Antigen Expression:HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 -
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9,10
D5S818: 11,14
D7S820: 8,14
THO1: 6,9.3
TPOX: 9,11
vWA: 18,19
Comments:The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.
DS-1 is IL-6 dependent.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: 55-X ).
Medium Renewal: Every 2 to 3 daysATCC Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) cells/ml. Maintain cultures between 1 X 10 (5) and 2 X 10 (6) cells/ml.Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Bioreactive Factors: Growth Factors: interleukin 6 (IL-6)
References: 22116: Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996
