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QPN1 12C9 (SCRF 43.2)
    QPN1 12C9 (SCRF 43.2)
  • 平臺(tái)編號(hào):bio-68612
  • 國(guó)際編號(hào):HB-9500?
  • 細(xì)胞信息: QPN1 12C9 (SCRF 43.2)
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
ATCC Number:HB-9500?
是否是腫瘤細(xì)胞:0
物種來源:小鼠
運(yùn)輸方式:凍存運(yùn)輸
數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG1
規(guī)格:1ml Designations: QPN1 12C9 (SCRF 43.2)
Depositors: ?Scripps Clinic and Research Foundation
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against p-nitroanaline amide derivatives
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a conjugate of keyhole limpet hemocyanin and a phosphonamidate compound.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with and catalyzes the hydrolysis of certain ester bonds.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:10 mM HEPES0.1 mM hypoxanthine0.032 mM thymidine2% bovine serum albumin1% Nutridoma-SP (Boehringer Mannheim Biochemicals, Catalog# 100-104)
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 21999: Lerner RA, et al. Molecules with antibody combining sites that exhibit catalytic properties. US Patent 5,126,258 dated Jun 30 1992

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