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平臺(tái)編號(hào)：bio-68616
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國(guó)際編號(hào)：CRL-2532?
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細(xì)胞信息： QNR/D
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
QNR/D
細(xì)胞類型：其他細(xì)胞類型
組織來源：neuroretina
是否是腫瘤細(xì)胞：0
物種來源：其他
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-2532?
年限：embryo, 7 days gestation
規(guī)格：1mg Designations: QNR/D
Depositors: ?B Pessac, D Trisler
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Coturnix coturnix japonica
Morphology:
Source: Tissue: neuroretina
Cell Type: neuronalinfected with Rous sarcoma virus mutant ts NY-68
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo, 7 days gestation
Comments:Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish the QNR ts NY-68 mixed cell line. [48961 ]
QNR ts NY-68 was subsequently cloned to establish the QNR/D cell line. The cells are routinely maintained at 38.5 to 39C. The permissive temperature for transformation is 36C. [48961 ]
Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. [48961 ]
The cell line displays properties of amacrine and/or ganglion cells.
QNR/D cells can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina. [48961 ]
QNR/D cells (ATCC CRL-2532 ) and QNR/K2 cell (ATCC CRL-2533 ) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. [48964 ]
In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. [48964 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Max Temperature: 39.0°C
Min Temperature: 38.5°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48961: Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691
48964: Trisler D, et al. Retinal engineering: engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804
51410: Cohen-Salmon M, et al. Characterization of the promoter of the human KAL gene, responsible for the X-chromosome-linked Kallmann syndrome. Gene 164: 235-242, 1995. PubMed: 7590336
組織來源：neuroretina
是否是腫瘤細(xì)胞：0
物種來源：其他
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-2532?
年限：embryo, 7 days gestation
規(guī)格：1mg Designations: QNR/D
Depositors: ?B Pessac, D Trisler
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Coturnix coturnix japonica
Morphology:
Source: Tissue: neuroretina
Cell Type: neuronalinfected with Rous sarcoma virus mutant ts NY-68
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo, 7 days gestation
Comments:Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish the QNR ts NY-68 mixed cell line. [48961 ]
QNR ts NY-68 was subsequently cloned to establish the QNR/D cell line. The cells are routinely maintained at 38.5 to 39C. The permissive temperature for transformation is 36C. [48961 ]
Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. [48961 ]
The cell line displays properties of amacrine and/or ganglion cells.
QNR/D cells can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina. [48961 ]
QNR/D cells (ATCC CRL-2532 ) and QNR/K2 cell (ATCC CRL-2533 ) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. [48964 ]
In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. [48964 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Max Temperature: 39.0°C
Min Temperature: 38.5°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 38.5?C.to 39?C
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 48961: Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691
48964: Trisler D, et al. Retinal engineering: engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804
51410: Cohen-Salmon M, et al. Characterization of the promoter of the human KAL gene, responsible for the X-chromosome-linked Kallmann syndrome. Gene 164: 235-242, 1995. PubMed: 7590336
