運(yùn)輸方式:凍存運(yùn)輸
生長狀態(tài):懸浮生長
是否是腫瘤細(xì)胞:0
物種來源:小鼠
ATCC Number:HB-261?
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG2b
數(shù)量:大量
規(guī)格:0.1ml Designations: FW3-218-1 [218]
Depositors: ?CR Mackay
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against sheep (ovine) alpha 4 integrin (VLA-4, CD49d)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with sheep afferent lymph lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with and precipitates sheep alpha 4 integrin.
It cross-reacts with bovine alpha 4 integrin, but not with the pig, rabbit or human counterparts.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22479: Mackay CR, et al. Tissue-specific migration pathways by phenotypically distinct subpopulations of memory T cells. Eur. J. Immunol. 22: 887-895, 1992. PubMed: 1372559