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ATCC Number：CRL-2842?
相關(guān)疾病：正常
運(yùn)輸方式：凍存運(yùn)輸
年限：1 day old newborn
是否是腫瘤細(xì)胞：0
物種來源：豬
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來源：腎皮質(zhì)
規(guī)格：1mg Designations: SK-RST
Depositors: ?RS Trowbridge
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Sus scrofa
Morphology:epithelial


Source: Organ: kidney, cortex
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1998
Applications:diagnostic studies
Cytogenetic Analysis:diploid
Age: 1 day old newborn
Gender: male
Comments:The cells are free from the classical swine fever viruses and porcine circoviruses as described in 9 CFR 113.46, 9 CFR 113.47 and 9 CFR 113.55. The cells may be used in diagnostic tests for swine diseases and for production of products for use in veterinary and human medicine.The SK-RST cells exhibited cytopathology after being infected with two serotypes of vesicular stomatitis virus, Newcastle disease virus, porcine parvovirus, pseudorabies virus, reovirus, transmissible gastroenteritis virus, bovine virus diarrhea, bovine adenovirus, bovine parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and with representatives of the A, O, C, SAT1, SAT2, SAT3 and Asia serotypes of foot-and-mouth disease virus. Classical swine fever virus (CSF)-infected SK-RST cells produced CSF antigen that was detectable using a CSF-antigen specific monoclonal antibody in an avidin-biotin complex immunohistochemistry assay. The SK-RST cells failed to exhibit cytopathology when exposed to porcine adenovirus, bluetongue virus, bovine respiratory syncytial virus, and bovine parvovirus. The SK-RST cells exhibited cytopathology after being infected with two serotypes of vesicular stomatitis virus, Newcastle disease virus, porcine parvovirus, pseudorabies virus, reovirus, transmissible gastroenteritis virus, bovine virus diarrhea, bovine adenovirus, bovine parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and with representatives of the A, O, C, SAT1, SAT2, SAT3 and Asia serotypes of foot-and-mouth disease virus. Classical swine fever virus (CSF)-infected SK-RST cells produced CSF antigen that was detectable using a CSF-antigen specific monoclonal antibody in an avidin-biotin complex immunohistochemistry assay. The SK-RST cells failed to exhibit cytopathology when exposed to porcine adenovirus, bluetongue virus, bovine respiratory syncytial virus, and bovine parvovirus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
   An inoculum of 5 X 10(3) to 7 X 10(3) viable cells/sq. cm is recommended.
6. Incubate cultures at 37?C.

Interval: Subculture when cells reach a concentration between 7 X 10(4) and 9 X 10(4) cells/sq. cm.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:8 is recommended
Medium Renewal: Every two to three days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 21 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
Erythrosin B vital stain solution:ATCC 30-2404
Trypan Blue vital stain solution:ATCC 30-2402