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生長狀態(tài)：懸浮生長
ATCC Number：TIB-12?
相關疾病：多發(fā)性骨髓瘤
細胞類型：B淋巴細胞
是否是腫瘤細胞：0
物種來源：小鼠
運輸方式：凍存運輸
數(shù)量：大量
品系：BALB/c
細胞形態(tài)：淋巴樣
免疫類型：IgG2a; kappa light chain
規(guī)格：1mg Designations: RPC5.4
Depositors: ?B Mohit
Isotype: IgG2a; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d
Cytogenetic Analysis:modal number = 60
Comments:The line was established from the RPC-5 mineral oil induced BALB/c plasmacytoma.
The cells are killed by 10 nM dexamethasone and are resistant to 0.1 mM 8-azaguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1113: Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030
1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
58048: Schwaber J. Human lymphocyte -- mouse myeloma somatic cell hybrids: selective hybrid formation. Somatic Cell Genet. 3: 295-302, 1977. PubMed: 305122