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平臺(tái)編號(hào)：bio-68645
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國際編號(hào)：CRL-1893?
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細(xì)胞信息： 372
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用及說明書：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
372
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG3
ATCC Number：CRL-1893?
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：1mg Designations: 372
Depositors: ?B Chesebro
Isotype: IgG3
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against p15E envelop antigen (ENV) protein of murine leukemia viruses (MuLV)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Spleen cells from a mouse inoculated with a B-tropic Friend murine leukemia virus (FrMuLV) were fused with NS-1 myeloma cells.
The antibody is highly specific for p15E protein from most MuLV.
The antibody efficiently immunoprecipitates p15E and works well in Western blotting assays for p15E.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1778: Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
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