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是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：淋巴樣
免疫類型：IgM
生長狀態(tài)：懸浮生長
數(shù)量：大量
運輸方式：凍存運輸
ATCC Number：HB-231?
規(guī)格：1mg Designations: 38.1
Depositors: ?PJ Martin
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human CD3
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human peripheral blood T lymphocytes.
Spleen cells were fused with NSI/1 myeloma cells.
The antibody reacts with the Tp19-29 antigen associated with human T cells (CD3).
The antibody moderately inhibits alloreactive cytotoxic T lymphocyte activity and is weakly mitogenic for peripheral blood lymphocytes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 24319: . Leucocyte typing: human leucocyte differentiation antig ens detected by monoclonal antibodies. New York: Springer-Verlag; 1984.