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Ep-16
    Ep-16
  • 平臺(tái)編號(hào):bio-68658
  • 國(guó)際編號(hào):HB-155?
  • 細(xì)胞信息: Ep-16
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
ATCC Number:HB-155?
數(shù)量:大量
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM; kappa light chain
規(guī)格:1mg Designations: Ep-16
Depositors: ?AW Hamburger
Isotype: IgM; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human epithelium
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a membrane enriched extract of human foreskin keratinocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
References: 1003: Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. PubMed: 2578308

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