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平臺(tái)編號(hào)：bio-68660
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國(guó)際編號(hào)：CRL-1644?
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細(xì)胞信息： SJK-287-38
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SJK-287-38
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：CRL-1644?
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100ml Designations: SJK-287-38
Depositors: ?D Korn
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2db
Comments:Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-179.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672
物種來(lái)源：小鼠
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：CRL-1644?
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100ml Designations: SJK-287-38
Depositors: ?D Korn
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2db
Comments:Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, but does not react with DNA polymerases beta and gamma.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-179.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672
