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細(xì)胞類型：其他細(xì)胞類型
品系：C3H/HeJ
數(shù)量：大量
器官來源：大腦
細(xì)胞形態(tài)：單核細(xì)胞/巨噬細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：小鼠
年限：10 days
ATCC Number：CRL-2467?
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：1.5ml Designations: EOC 2
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:macrophage


Source: Organ: brain
Strain: C3H/HeJ
Cell Type: microglia;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression:CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD86 (B7.2) +; CD45 +, Ly-6C +, F4/80 +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR + [39974 ]
Age: 10 days
Gender: female
Comments:This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse. [39974 ]
Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping.
The cell line is dependent on colony stimulating factor 1 (CSF-1).
The cells exhibit phagocytic activity.
Unlike EOC 13.31 (ATCC CRL-2468 ) and EOC 20 (ATCC CRL-2469 ), these cells do not constitutively express high levels of major histocompatibility complex (MHC) class II antigens and expression is not upregulated by recombinant murine interferon-gamma.
The cells may be used to characterize the role of brain macrophages. C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard 75% of the media.
2. Scrape off the attached cells with a cell scraper.
3. Add appropriate aliquots of cell suspension to new culture vessels.
4. Incubate cultures at 37?C.
   
   Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
   Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530
39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775