ATCC Number:HB-8180?
數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG2a
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
規(guī)格:100ug Designations: 39-S
Depositors: ?The United States of America
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against herpes simplex virus type 1 (HSV-1) internal capsid protein 8 (ICP 8)
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Tumorigenic:Yes
Comments:Animals were immunized with HSV-1.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody is type specific for HSV-1 but does not neutralize the virus.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15% -OR- RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1178: Showalter SD, et al. Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4. Infect. Immun. 34: 684-692, 1981. PubMed: 6277788
3544: Zweig M, et al. Monoclonal antibodies to herpes simplex virus type 1 polypeptides. US Patent 4,572,896 dated Feb 25 1986