是否是腫瘤細(xì)胞：0
物種來源：小鼠
器官來源：胸腺
ATCC Number：TIB-181?
相關(guān)疾?。毫馨土?br />
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
運輸方式：凍存運輸
生長狀態(tài)：懸浮生長
品系：C57BL/6
規(guī)格：10ml Designations: EL4.IL-2
Depositors: ?JJ Farrar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Organ: thymus
Strain: C57BL/6
Disease: lymphoma
Cellular Products:interleukin-2 (interleukin 2, IL-2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This is a subline of EL4 (ATCC TIB-39 ) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).
The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 to 2 X 10(6) viable cells/ml..
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2040
parental cell line:ATCC TIB-39
References: 1142: Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789
21883: . Cancer therapeutics. New York: Raven Press; 1981.
23210: Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931
