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生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
品系：C57BL/6N
ATCC Number：TIB-41?
相關(guān)疾?。毫馨土?br /> 細(xì)胞類型：T淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：10ml Designations: EL4.BU.1.OUAr.1.1
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2b; Thy-1.2
Comments:This line was derived from the EL4 cell line (see ATCC TIB-39 ).
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22247: Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.