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平臺(tái)編號(hào)：bio-68703
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國際編號(hào)：CRL-1889?
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細(xì)胞信息： 34
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
34
生長狀態(tài)：懸浮生長
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b , IgG2b
ATCC Number：CRL-1889?
數(shù)量：大量
規(guī)格：100ug Designations: 34
Depositors: ?B Chesebro
Isotype: IgG2b , IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against p15 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells.
The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses.
The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1778: Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b , IgG2b
ATCC Number：CRL-1889?
數(shù)量：大量
規(guī)格：100ug Designations: 34
Depositors: ?B Chesebro
Isotype: IgG2b , IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against p15 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Spleen cells from a mouse recovering from erythroleukemia induced by a B-tropic Friend murine leukemia virus were fused with NS-1 myeloma cells.
The antibody reacts with the p15 protein from several MuLV including Friend, Moloney, Rauscher, amphotropic and Spleen Focus Forming (SFFV) viruses.
The antibody reacts with infected cells after acetone fixation and immunoprecipitates p15.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1778: Chesebro B, et al. Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112: 131-144, 1981. PubMed: 6787798
1779: Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011
