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平臺(tái)編號(hào)：bio-68704
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國(guó)際編號(hào)：CRL-1149?
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細(xì)胞信息： El Don
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
El Don
ATCC Number：CRL-1149?
相關(guān)疾?。浩渌膊?br /> 年限：31 years
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來源：皮膚
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：1000ml Designations: El Don
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: Ehlers-Danlos syndrome, type VII (arthrocalasis type)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 31 years
Gender: female
Comments:The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
References: 22242: Lichtenstein JR, et al. Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738
相關(guān)疾?。浩渌膊?br /> 年限：31 years
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來源：皮膚
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：1000ml Designations: El Don
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: Ehlers-Danlos syndrome, type VII (arthrocalasis type)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 31 years
Gender: female
Comments:The tissue donor suffered from type VII Ehlers-Danlos syndrome (arthrocalasis type), and was patient No. 2 in Lichtenstein, JR, et al. Science 182:298, 1973.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
References: 22242: Lichtenstein JR, et al. Defect in conversion of procollagen to collagen in a form of Ehlers-Danlos syndrome. Science 182: 298-300, 1973. PubMed: 4742738
