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生長狀態(tài)：懸浮生長
器官來源：脾
細胞形態(tài)：單核細胞/巨噬細胞
是否是腫瘤細胞：0
物種來源：人
ATCC Number：CRL-9854?
數(shù)量：大量
運輸方式：凍存運輸
規(guī)格：0.5ml Designations: EL 1
Depositors: ?E.I. du Pont de Nemours & Co.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:monocyte/macrophage


Source: Organ: spleen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The EL 1 cell line was established from cryopreserved human spleen cells.
The cells tend to grow as single cells but may form small nonadherent clusters.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; calf serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml)
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
References: 26300: Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995