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平臺(tái)編號(hào)：bio-68719
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國際編號(hào)：CRL-2108
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細(xì)胞信息： EHS*
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
EHS*
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：0
物種來源：小鼠
品系：C57BL
運(yùn)輸方式：凍存運(yùn)輸
組織來源：connective tissue
ATCC Number：CRL-2108?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.5ml Designations: EHS
Depositors: ?H Kleinman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: connective tissue
Strain: C57BL
Disease: sarcoma
Cellular Products:laminin; collagen IV; entactin; heparin sulfate proteoglycan
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:EHS (Englebreth-Holm-Swarm) is a sarcoma carried as a transplantable tumor in C57BL mice.
It produces all the basement membrane components (laminin, collagen IV, entactin, and heparan sulfate proteoglycan).
The EHS sarcoma is a source of Matrigel, an extract that stimulates cell differentiation.
Matrigel is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan.
The EHS sarcoma is a rich source of both individual basement membrane components and Matrigel.
The ATCC stocks were established from tumors grown in C57BL/6J mice.
Propagation: ATCC complete growth medium: Duration: These cell are propagated in C57BL/6 mice.
Subculturing: Protocol: Harvest tumor from one mouse, mince and inject intramuscularly (over the hindlimb) into 3 to 10 C57BL/6 mice. Examine the mice daily for tumor progression, and harvest tumors (usually about 14 to 21 days after inoculation).
Preservation: Freeze medium: Ham's F12K medium, 80%; fetal bovine serum; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
References: 22608: Kleinman HK, et al. Basement membrane complexes with biological activity. Biochemistry 25: 312-318, 1986. PubMed: 2937447
22966: . . J. Tissue Culture Methods 16: 227-230, 1994.
23396: Timpl R, et al. Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254: 9933-9937, 1979. PubMed: 114518
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：0
物種來源：小鼠
品系：C57BL
運(yùn)輸方式：凍存運(yùn)輸
組織來源：connective tissue
ATCC Number：CRL-2108?
相關(guān)疾?。浩渌膊?br /> 規(guī)格：0.5ml Designations: EHS
Depositors: ?H Kleinman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: connective tissue
Strain: C57BL
Disease: sarcoma
Cellular Products:laminin; collagen IV; entactin; heparin sulfate proteoglycan
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:EHS (Englebreth-Holm-Swarm) is a sarcoma carried as a transplantable tumor in C57BL mice.
It produces all the basement membrane components (laminin, collagen IV, entactin, and heparan sulfate proteoglycan).
The EHS sarcoma is a source of Matrigel, an extract that stimulates cell differentiation.
Matrigel is rich in laminin, growth factors, entactin/nidogen, type IV collagen, and heparan sulfate proteoglycan.
The EHS sarcoma is a rich source of both individual basement membrane components and Matrigel.
The ATCC stocks were established from tumors grown in C57BL/6J mice.
Propagation: ATCC complete growth medium: Duration: These cell are propagated in C57BL/6 mice.
Subculturing: Protocol: Harvest tumor from one mouse, mince and inject intramuscularly (over the hindlimb) into 3 to 10 C57BL/6 mice. Examine the mice daily for tumor progression, and harvest tumors (usually about 14 to 21 days after inoculation).
Preservation: Freeze medium: Ham's F12K medium, 80%; fetal bovine serum; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
References: 22608: Kleinman HK, et al. Basement membrane complexes with biological activity. Biochemistry 25: 312-318, 1986. PubMed: 2937447
22966: . . J. Tissue Culture Methods 16: 227-230, 1994.
23396: Timpl R, et al. Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254: 9933-9937, 1979. PubMed: 114518
