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平臺編號：bio-68733
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國際編號：CCL-192?
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細(xì)胞信息： RFL-6
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
RFL-6
器官來源：肺
ATCC Number：CCL-192?
相關(guān)疾?。赫?br /> 品系：Sprague-Dawley
年限：18 days gestation
運輸方式：凍存運輸
細(xì)胞形態(tài)：成纖維樣
細(xì)胞類型：成纖維細(xì)胞
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：大鼠
數(shù)量：大量
規(guī)格：0.5ml Designations: RFL-6
Depositors: ?ML Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: lung
Strain: Sprague-Dawley
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 18 days gestation
Propagation: ATCC complete growth medium: Ham's F12K medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit for 10 to 12 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 6 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
ATCC Number：CCL-192?
相關(guān)疾?。赫?br /> 品系：Sprague-Dawley
年限：18 days gestation
運輸方式：凍存運輸
細(xì)胞形態(tài)：成纖維樣
細(xì)胞類型：成纖維細(xì)胞
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：大鼠
數(shù)量：大量
規(guī)格：0.5ml Designations: RFL-6
Depositors: ?ML Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:fibroblast
Source: Organ: lung
Strain: Sprague-Dawley
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 18 days gestation
Propagation: ATCC complete growth medium: Ham's F12K medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit for 10 to 12 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 6 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
