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平臺(tái)編號(hào)：bio-68739
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國(guó)際編號(hào)：CRL-2672?
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細(xì)胞信息： 240-13D10
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
240-13D10
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：CRL-2672?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 and IgG2b
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: 240-13D10
Depositors: ?National Cancer Institute
Isotype: IgG1 and IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected.
In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.
The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：CRL-2672?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 and IgG2b
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: 240-13D10
Depositors: ?National Cancer Institute
Isotype: IgG1 and IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected.
In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.
The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
