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平臺(tái)編號(hào)：bio-68743
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國際編號(hào)：CRL-1700?
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細(xì)胞信息： SCP
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
SCP
ATCC Number：CRL-1700?
運(yùn)輸方式：凍存運(yùn)輸
組織來源：choroid plexus
器官來源：大腦
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：綿羊
生長狀態(tài)：貼壁生長
規(guī)格：0.5ml Designations: SCP
Depositors: ?W Siegel, T Booker
Biosafety Level:2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Ovis aries
Morphology:
Source: Organ: brain
Tissue: choroid plexus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility:Visna virus (strain 1514)
Comments:The line is positive for bovine viral diarrhea virus (BVD).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528
運(yùn)輸方式：凍存運(yùn)輸
組織來源：choroid plexus
器官來源：大腦
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：綿羊
生長狀態(tài)：貼壁生長
規(guī)格：0.5ml Designations: SCP
Depositors: ?W Siegel, T Booker
Biosafety Level:2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Ovis aries
Morphology:
Source: Organ: brain
Tissue: choroid plexus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility:Visna virus (strain 1514)
Comments:The line is positive for bovine viral diarrhea virus (BVD).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528
