運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞類(lèi)型:B淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來(lái)源:人
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
ATCC Number:CCL-104?
相關(guān)疾?。浩渌膊?br />
細(xì)胞形態(tài):淋巴樣
數(shù)量:大量
器官來(lái)源:外周血
年限:20 years
規(guī)格:0.5ml Designations: E.H. IV (Elaine IV)
Depositors: ?W Henle
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: infectious mononucleosis
Cell Type: B lymphoblast;
Cellular Products:Epstein-Barr virus (EBV)
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Virus Resistance:partially resistant to poliovirus and vesicular stomatitis viruses
Isoenzymes: G6PD, B
Age: 20 years
Gender: female
Ethnicity: Caucasian
Comments:The cells carry and produce Epstein-Barr virus (EBV).
EBNA positive.
Biological Safety CLASS II.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cultures can be maintained by addition of fresh medium or by replacement of medium by sedimentation. Establish new cultures by seeding in fresh medium at 5 X 10 exp5 viable cells/ml.
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
26274: Niederman JC, et al. Infectious mononucleosis. Clinical manifestations in relation to EB virus antibodies. JAMA 203: 205-209, 1968. PubMed: 4864269