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平臺(tái)編號(hào)：bio-68757
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國(guó)際編號(hào)：CRL-1404
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細(xì)胞信息： SC-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu) 說(shuō)明書(shū)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SC-1
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來(lái)源：胚胎
ATCC Number：CRL-1404?
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
年限：embryo
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5ml Designations: SC-1
Depositors: ?JW Hartley
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:Initial cultures from a thawed ampule may take 10 to 12 days to become confluent.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22696: Hartley JW, Rowe WP. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65: 128-134, 1975. PubMed: 167514
