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首頁>細胞CELL>ATCC普通細胞>E (Ehrlich-Lettre ascites)
E (Ehrlich-Lettre ascites)
    E (Ehrlich-Lettre ascites)
  • 平臺編號:bio-68759
  • 國際編號:CCL-77?
  • 細胞信息: E (Ehrlich-Lettre ascites)
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

是否是腫瘤細胞:0
物種來源:小鼠
運輸方式:凍存運輸
生長狀態(tài):貼壁生長
ATCC Number:CCL-77?
數(shù)量:大量
細胞形態(tài):上皮樣
規(guī)格:0.5ml Designations: E (Ehrlich-Lettre ascites)
Depositors: ?CW Boone
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 3
Comments:Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: NCTC 135 medium, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure can be used.
References: 22170: Boone C, et al. Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passages. J. Natl. Cancer Inst. 34: 725-740, 1965. PubMed: 5829538

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