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器官來源：外周血
數(shù)量：大量
細胞形態(tài)：單核細胞/巨噬細胞
生長狀態(tài)：懸浮生長
ATCC Number：CRL-9855?
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：人
運輸方式：凍存運輸
規(guī)格：0.5ml Designations: SC
Depositors: ?E.I. du Pont de Nemours & Co.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:monocyte/macrophage


Source: Organ: peripheral blood
Cell Type: monocyte/macrophage
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The SC cell line was established from human peripheral blood mononuclear cells.
The cells grow as single cells in suspension, and have a granular appearance.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium.
Interval: Maintain the cell concentration between 2 X 10(5) and 1 X 10 (6) cells/ml)
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 26300: Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995