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平臺(tái)編號(hào)：bio-68765
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國(guó)際編號(hào)：TIB-69?
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細(xì)胞信息： RAW 309 Cr.1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
RAW 309 Cr.1
細(xì)胞類(lèi)型：其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：TIB-69?
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：0.5ml Designations: RAW 309 Cr.1
Depositors: ?WC Raschke
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Cell Type: monocyte/macrophage
Cellular Products:Abelson murine leukemia virus (infectious); lysozyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; H-2b
Comments:RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51 ).
The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells will pinocytose neutral red and phagocytose latex beads and zymosan.
They will also lyse antibody coated sheep erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：TIB-69?
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：0.5ml Designations: RAW 309 Cr.1
Depositors: ?WC Raschke
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:
Source: Cell Type: monocyte/macrophage
Cellular Products:Abelson murine leukemia virus (infectious); lysozyme
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; H-2b
Comments:RAW 309 Cr.1 was derived from the same tumor as RAW 309F.1.1 (see ATCC TIB-51 ).
The cells are negative for surface immunoglobulin (sIg-) Thy-1.2 (Thy-1.2-) and Ia (Ia-).
The cells will pinocytose neutral red and phagocytose latex beads and zymosan.
They will also lyse antibody coated sheep erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
