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數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG2a
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：TIB-119?
規(guī)格：0.5ml Designations: RAN-2 [alpha RAN-2, anti RAN-2]
Depositors: ?PF Bartlett, M Raff
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with enriched preparations of rat astrocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with cultured neurons, oligodendrocytes, Schwann cells, microglia or various non-neuronal cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 902: Bartlett PF, et al. Rat neural antigen-2 (RAN-2): a cell surface antigen on astrocytes, ependymal cells, Muller cells and lepto-meninges defined by a monoclonal antibody. Brain Res. 204: 339-351, 1980. PubMed: 7006734