-
平臺(tái)編號(hào)：bio-68789
-
國(guó)際編號(hào)：TIB-30?
-
細(xì)胞信息： S49.1TB.2
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
S49.1TB.2
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
細(xì)胞類(lèi)型：T淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：TIB-30?
相關(guān)疾?。毫馨土?br /> 運(yùn)輸方式：凍存運(yùn)輸
品系：BALB/c
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
規(guī)格：0.5ml Designations: S49.1TB.2
Depositors: ?A Harris
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; Thy-1.2; TL
Comments:This line was derived from S49.1 (ATCC TIB-28 ) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230
細(xì)胞類(lèi)型：T淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
ATCC Number：TIB-30?
相關(guān)疾?。毫馨土?br /> 運(yùn)輸方式：凍存運(yùn)輸
品系：BALB/c
細(xì)胞形態(tài)：淋巴樣
數(shù)量：大量
規(guī)格：0.5ml Designations: S49.1TB.2
Depositors: ?A Harris
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; Thy-1.2; TL
Comments:This line was derived from S49.1 (ATCC TIB-28 ) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.
Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928
22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22241: Schiller JG, et al. Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230
