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平臺編號：bio-68795
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國際編號：TIB-146?
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細(xì)胞信息： RA3-3A1/6.1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
RA3-3A1/6.1
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：大鼠
ATCC Number：TIB-146?
數(shù)量：大量
規(guī)格：0.5ml Designations: RA3-3A1/6.1
Depositors: ?RL Coffman
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：大鼠
ATCC Number：TIB-146?
數(shù)量：大量
規(guī)格：0.5ml Designations: RA3-3A1/6.1
Depositors: ?RL Coffman
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
