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ATCC Number：HB-9255?
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
生長狀態(tài)：懸浮生長
數(shù)量：大量
規(guī)格：0.5ml Designations: S5
Depositors: ?Sloan-Kettering Institute for Cancer Research
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; gp120 glycoprotein
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with the SK-MG-17 human astrocytoma cell line.
The antibody reacts with a glycoprotein (gp120) of relative molecular weight 120000 daltons.
The antigen is expressed on cultured human fibroblasts and sarcomas.
In normal tissues, S5 antigen expression is limited to visceral smooth muscle.
In a survey of tumors, S5 staining was limited to a subset of leiomyosarcomas and the staining was generally weaker than that with normal smooth muscle.
Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21984: Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991
23062: Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731