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平臺(tái)編號(hào)：bio-68804
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國際編號(hào)：CRL-11516?
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細(xì)胞信息： 2.040 pRSV-T
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
2.040 pRSV-T
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
組織來源：cornea
器官來源：眼
生長狀態(tài)：貼壁生長
ATCC Number：CRL-11516?
數(shù)量：大量
規(guī)格：0.5ml Designations: 2.040 pRSV-T
Depositors: ?T Walker, Gillette Medical Evaluation Laboratories
Biosafety Level:2 [Cells contain SV-40 and Rous Sarcoma viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: eye
Tissue: cornea
Cell Type: epithelial transfected with a plasmid containing the SV40 early region
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:A primary culture of normal corneal epithelium was immortalized by transfection with plasmid pRSV-T using lipofectamine overnight at 37C. pRSV-T contains the SV40 early region genes and the Rous Sarcoma virus long terminal repeat.
Propagation: ATCC complete growth medium: Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 5 ng/ml human recombinant EGF, 0.05 mg/ml bovine pituitary extract, 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
Temperature: 37.0°C
Subculturing: Protocol: The cells should be subcultured before reaching confluence.
Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Neutralize the trypsin with fresh growth medium, centrifuge at 300 x g for 8 minutes, resuspend the pellet in fresh growth medium and dispense into new COATED flasks. Flasks used to propagate these cells must be coated (2 hours at 37C) with 0.01 mg/ml bovine serum albumin, 0.01 mg/ml fibronectin and 0.03 mg/ml bovine collagen type I .
Incubate at 37C with 5% CO2
Subcultivation Ratio: Subculture at 1:3 when 80% confluent.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 82.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
References: 34094: Walker TL, Kahn CR. Human corneal epithelial cell lines with extended lifespan. US Patent 5,672,498 dated Sep 30 1997
細(xì)胞形態(tài)：上皮樣
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
組織來源：cornea
器官來源：眼
生長狀態(tài)：貼壁生長
ATCC Number：CRL-11516?
數(shù)量：大量
規(guī)格：0.5ml Designations: 2.040 pRSV-T
Depositors: ?T Walker, Gillette Medical Evaluation Laboratories
Biosafety Level:2 [Cells contain SV-40 and Rous Sarcoma viral sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: eye
Tissue: cornea
Cell Type: epithelial transfected with a plasmid containing the SV40 early region
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:A primary culture of normal corneal epithelium was immortalized by transfection with plasmid pRSV-T using lipofectamine overnight at 37C. pRSV-T contains the SV40 early region genes and the Rous Sarcoma virus long terminal repeat.
Propagation: ATCC complete growth medium: Keratinocyte-Serum Free medium (GIBCO-BRL 17005-042) with 5 ng/ml human recombinant EGF, 0.05 mg/ml bovine pituitary extract, 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
Temperature: 37.0°C
Subculturing: Protocol: The cells should be subcultured before reaching confluence.
Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Neutralize the trypsin with fresh growth medium, centrifuge at 300 x g for 8 minutes, resuspend the pellet in fresh growth medium and dispense into new COATED flasks. Flasks used to propagate these cells must be coated (2 hours at 37C) with 0.01 mg/ml bovine serum albumin, 0.01 mg/ml fibronectin and 0.03 mg/ml bovine collagen type I .
Incubate at 37C with 5% CO2
Subcultivation Ratio: Subculture at 1:3 when 80% confluent.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 82.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
References: 34094: Walker TL, Kahn CR. Human corneal epithelial cell lines with extended lifespan. US Patent 5,672,498 dated Sep 30 1997
