ATCC Number:CRL-1289?
數(shù)量:大量
相關(guān)疾病:馬凡氏綜合征
是否是腫瘤細(xì)胞:0
物種來源:人
器官來源:皮膚
年限:6 years
細(xì)胞形態(tài):成纖維樣
運(yùn)輸方式:凍存運(yùn)輸
生長(zhǎng)狀態(tài):貼壁生長(zhǎng)
規(guī)格:0.5ml Designations: Ra Lot
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: Marfan syndrome
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Age: 6 years
Gender: male
Comments:The tissue donor suffered from a severe form of Marfan syndrome.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.