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S49.1G.3
    S49.1G.3
  • 平臺編號:bio-68811
  • 國際編號:TIB-34?
  • 細(xì)胞信息: S49.1G.3
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

數(shù)量:大量
生長狀態(tài):懸浮生長
細(xì)胞類型:T淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來源:小鼠
品系:BALB/c
ATCC Number:TIB-34?
相關(guān)疾?。毫馨土?br /> 細(xì)胞形態(tài):淋巴樣
運(yùn)輸方式:凍存運(yùn)輸
規(guī)格:0.5ml Designations: S49.1G.3
Depositors: ?A Harris
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2d; Thy-1.2; TL
Comments:This variant of S49.1 (ATCC TIB-28 ) was selected for resistance to 1 mM 6-thioguanine.
The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

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