數(shù)量:大量
生長狀態(tài):懸浮生長
細(xì)胞類型:T淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來源:小鼠
品系:BALB/c
ATCC Number:TIB-34?
相關(guān)疾?。毫馨土?br />
細(xì)胞形態(tài):淋巴樣
運(yùn)輸方式:凍存運(yùn)輸
規(guī)格:0.5ml Designations: S49.1G.3
Depositors: ?A Harris
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
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Antigen Expression:H-2d; Thy-1.2; TL
Comments:This variant of S49.1 (ATCC TIB-28 ) was selected for resistance to 1 mM 6-thioguanine.
The cells are sensitive to 0.01 mM thymidine, 0.001 mM hydrocortisone and 5 mcg/ml phytohemagglutinin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928