運輸方式:凍存運輸
器官來源:脾
數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
ATCC Number:CRL-9300?
細(xì)胞類型:B淋巴細(xì)胞
是否是腫瘤細(xì)胞:0
物種來源:人
生長狀態(tài):懸浮生長
規(guī)格:0.5mg Designations: 20H11
Depositors: ?Genetic Systems Corp.
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed
Cellular Products:immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus.
The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7.
The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Doubling Time: 24 to 36 hrs
References: 3871: Rosok MJ, Lostrom ME. Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989