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S1A(Thy-1-b)
    S1A(Thy-1-b)
  • 平臺編號:bio-68827
  • 國際編號:TIB-231?
  • 細胞信息: S1A(Thy-1-b)
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

運輸方式:凍存運輸
ATCC Number:TIB-231?
相關(guān)疾?。毫馨土?br /> 數(shù)量:大量
細胞形態(tài):淋巴樣
細胞類型:T淋巴細胞
是否是腫瘤細胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
品系:BALB/c
規(guī)格:0.5mg Designations: S1A(Thy-1-b)
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Disease: lymphoma
Strain: BALB/c
Cell Type: T lymphocyte;
Cellular Products:The cells secrete a mutant Thy-1 glycoprotein.
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27 ).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1807: Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421

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