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細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgM; kappa light chain
數(shù)量：大量
ATCC Number：TIB-112?
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.5ml Designations: S-S.3
Depositors: ?WC Raschke
Isotype: IgM; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against sheep red blood cell (SRBC, erythrocyte)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with SRBC.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
In 1986 this line was recloned at the ATCC from the original deposit.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.