生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
ATCC Number:CRL-1812?
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類(lèi)型:IgG1
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
規(guī)格:0.5mg Designations: 2E10-H2
Depositors: ?CA Whetstone
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against canine adenovirus type 1 (CAV-1)
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Tumorigenic:Yes
Comments:Animals were immunized with purified CAV-1 (Mirandola strain).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to and neutralizes CAV-1 and is type specific.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22210: Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371
23146: Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549