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2E10-H2
    2E10-H2
  • 平臺(tái)編號(hào):bio-68856
  • 國(guó)際編號(hào):CRL-1812?
  • 細(xì)胞信息: 2E10-H2
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
ATCC Number:CRL-1812?
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞形態(tài):淋巴樣
免疫類(lèi)型:IgG1
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
規(guī)格:0.5mg Designations: 2E10-H2
Depositors: ?CA Whetstone
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against canine adenovirus type 1 (CAV-1)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with purified CAV-1 (Mirandola strain).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to and neutralizes CAV-1 and is type specific.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22210: Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371
23146: Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549

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