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平臺編號：bio-68859
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國際編號：HB-8995?
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細胞信息： 2C5-6
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
2C5-6
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
數(shù)量：大量
ATCC Number：HB-8995?
細胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：0.5mg Designations: 2C5-6
Depositors: ?G Schwartz
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against cholesterol
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. [57615 ]
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. [57615 ]
The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.
A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 49063: Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660
57615: Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
數(shù)量：大量
ATCC Number：HB-8995?
細胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：0.5mg Designations: 2C5-6
Depositors: ?G Schwartz
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against cholesterol
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. [57615 ]
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody detects cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. [57615 ]
The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.
A feeder layer of mouse peritoneal macrophages or mouse fibroblasts (see ATCC 56-X, irradiated STO cells) is require to propagate this cell line.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 49063: Clarke MS, et al. In situ localization of cholesterol in skeletal muscle by use of a monoclonal antibody. J. Appl. Physiol. 89: 731-741, 2000. PubMed: 10926660
57615: Swartz GM Jr., et al. Antibodies to cholesterol. Proc. Natl. Acad. Sci. USA 85: 1902-1906, 1988. PubMed: 3162316
